23. mai 2019

/CropBox [0 -5.66927 595.276001 785.197021] /SMask 419 0 R /Kids [15 0 R] endobj >> /D [27 0 R /FitR 307 232 545 232] >> /Parent 10 0 R /D [19 0 R /FitR 307 148 545 148] /TrimBox [0 -5.66927 595.276001 785.197021] 133 0 obj << Y1 - 2012. 3B2 Total Publishing System 8.07e/W Unicode of embryos, ranging from insects to vertebrates (e.g. 34 0 obj << (CR63) 90 0 R (CR64) 91 0 R (CR65) 92 0 R (CR66) 93 0 R] endobj /D [28 0 R /FitR 307 114 545 114] endobj endobj Recently we showed the critical role of two members of the Bcl-2 family of proteins during early zebrafish development. Asynchrony arises at the earliest stages, and it becomes more pro- nounced as time passes. USA.gov. /Annots [214 0 R 215 0 R 216 0 R 217 0 R 218 0 R 219 0 R 220 0 R 221 0 R 222 0 R 223 0 R << /Contents 241 0 R << 106 0 obj The gain, saturation, exposure, and lighting levels were identical for each imaging procedure. >> The data for these analyses were generated by exposing zebrafish embryos to a library of compounds provided by the NTP in DT and NT assays. AU - Zhu, Xiaoshan. Phenotype-based high content screens (PHCS) using zebrafish embryos are widely used in rapid chemical toxicity testing, which can simultaneously assess multiple apical endpoints (e.g., mortality and malformation effects). We then give an overview of new FCS modalities that are designed to cope with emerging challenges in intracellular applications, e.g. endobj endobj Zebrafish and humans share 70% of the same genes. Cell microinjection is a technique of precise delivery of substances into cells and is widely used for studying cell transfection, signaling pathways, and organelle functions. Here, we investigated the applicability of pollutant injection into the yolk of early zebrafish (Danio rerio) … /D [29 0 R /FitR 52 124 290 124] >> /D [29 0 R /FitR 52 164 290 164] /Rotate 0 /Subtype /XML /D [24 0 R /FitR 0 786 596 0] Cell microinjection is a technique of precise delivery of substances into cells and is widely used for studying cell transfection, signaling pathways, and organelle functions. Methods. /G3 173 0 R /D [28 0 R /FitR 307 543 545 543] This shows manual dechorionating (removing the shell) from 1 day old zebrafish embryos. 388 0 R 389 0 R] endobj << 26 0 obj endobj >> /Contents 323 0 R /D [27 0 R /FitR 307 622 545 622] /F (folder.joboptions) 143 0 obj >> >> /D [28 0 R /FitR 307 662 545 662] The authors declare no competing interests. endobj /Metadata 2 0 R However, factors such as the small cell size, high cell deformation … Zebrafish Maintenance Zebrafish were maintained essentially as described in Westerfield 6. In the area of toxicology, a number of in vivo assays based on zebrafish embryos and larvae are now available to examine the potential toxicity of compounds early in the drug discovery process [7,8], as /MediaBox [0 0 594.95996 840.95996] 108 0 obj Fig. /D [29 0 R /FitR 52 553 290 553] endobj 166 0 obj /Annots [393 0 R 394 0 R 395 0 R 396 0 R 397 0 R 398 0 R 399 0 R] Early zebrafish embryos are less than 1 mm in diameter, allowing several embryos to fit easily in a single well of a 384-well plate. >> >> /D [28 0 R /FitR 307 264 545 264] endobj 2018 Nov 1;150:32-41. doi: 10.1016/j.ymeth.2018.07.004. /Length 3332 /ca 1 >> Fig. endobj 150 0 obj >> Figure 2. >> (CR54) 80 0 R (CR55) 81 0 R (CR56) 82 0 R (CR57) 83 0 R (CR58) 84 0 R Anom. endobj endobj << The epigenetics of zebrafish embryos and different tissues in adult zebrafish are modulated in development [138, 149, 150] and in cancer [145, 151]. Swaim LE, Connolly LE, Volkman HE, Humbert O, Born DE, Ramakrishnan L. Infect Immun. AU - Chang, Yung. endobj Y1 - 2012. /TrimBox [0 -5.66927 595.276001 785.197021] 146 0 obj /D [19 0 R /FitR 52 334 290 334] >> >> >> endobj To increase total egg pro - duction, fish can be set up in a ratio of two females to one male if desired. /Parent 10 0 R 153 0 obj << /D [28 0 R /FitR 307 493 545 493] Mating and egg harvesting procedure for zebrafish: 1. << 27 0 obj endobj 2014;1124:219–233. The zebrafish (Danio rerio) is a freshwater fish belonging to the minnow family of the order Cypriniformes.Native to South Asia, it is a popular aquarium fish, frequently sold under the trade name zebra danio (and thus often called a "tropical fish" although both tropical and subtropical).. 23 0 obj /D [25 0 R /FitR 307 546 545 546] /First 9 0 R >> << >> 2014-11-13T14:02:24+01:00 303 0 R 304 0 R 305 0 R 306 0 R 307 0 R 308 0 R 309 0 R 310 0 R 311 0 R 312 0 R More recently, the zebrafish embryo has been applied … /MediaBox [0 -5.66927 595.276001 785.197021] endobj /Annots [283 0 R 284 0 R 285 0 R 286 0 R 287 0 R 288 0 R 289 0 R 290 0 R 291 0 R 292 0 R VEGF Inhibitor Treatment 2017;12:379–389. /BM /Normal ZebraBeat: a flexible platform for the analysis of the cardiac rate in zebrafish embryos… /Kids [10 0 R 11 0 R] DNA methylation patterns are reprogrammed in pre-implantation zebrafish embryos, as in mammals . Zebrafish embryos provide a unique opportunity to visualize complex biological processes, yet conventional imaging modalities are unable to access intricate biomolecular information without compromising the integrity of the embryos. endobj >> 10 0 obj /D [27 0 R /FitR 307 322 545 322] << /D [29 0 R /FitR 307 264 545 264] >> 22 0 obj /D [28 0 R /FitR 307 134 545 134] 53 0 obj 2012;485:471–477. >> (HypASec5) 134 0 R (HypAbs1) 135 0 R (HypBib1) 136 0 R (HypFig1) 137 0 R (HypFig2) 138 0 R Drug Discov. /D [28 0 R /FitR 52 303 290 303] From effects of nanosized HA, SiO(2) and TiO(2) particles on the zebrafish embryos development, they were adsorbed on the membrane surface confirmed by the electronic scanning microscopy. 62 0 obj endobj x��yp��}�h i�f&i2�2S�4d�IҤM�I�v��1M�6�N2iC�M�dhJ��Ʒ�|��`cc|b�ԧ$�>�u�CƦHZ�m�p��:˫߻Z��~�Ϯ���}w�w?�~��_�R Ѕ7����������f���������R�:f���z����\i�t�*\�n��]߸�Kw�7�Q޺63�p�:r�d�����k�-�U����t���!�z��1�l�k�k��͍h�mlm=�N���.l��6�k��j���ce�p��p�� ? << application/pdf >> /D [29 0 R /FitR 52 483 290 483] 2- Place the embryos at the expected developmental stage into a pre-chilled dounce glass and add 1 ml of ice-cold MB buffer. /D [29 0 R /FitR 52 702 290 702] >> /Rotate 0 Fig. 140 0 obj endobj /XObject << /Resources 339 0 R Fig. /D [19 0 R /FitR 307 383 545 383] /Prev 172 0 R /Annots [356 0 R 357 0 R 358 0 R 359 0 R 360 0 R 361 0 R 362 0 R 363 0 R 364 0 R 365 0 R >> From effects of nanosized HA, SiO(2) and TiO(2) particles on the zebrafish embryos development, they were adsorbed on the membrane surface confirmed by the electronic scanning microscopy. /Parent 10 0 R 2. Preparation of zebrafish embryos extracts. Zebrafish embryos were kept in a 28 °C incubator in E3 media (5 mM NaCl, 0.33 mM MgSO 4, 0.33 mM CaCl 2, 0.17 mM KCl, and 0.1% methylene blue). 366 0 R 367 0 R 368 0 R 369 0 R 370 0 R 371 0 R 372 0 R 373 0 R 374 0 R] /Thumb 413 0 R AU - Chen, Yongsheng. 234 0 R 235 0 R 236 0 R 237 0 R 238 0 R 239 0 R 240 0 R] endobj /D [19 0 R /FitR 307 383 545 383] /Limits [(CR67) (Section15)] endobj /Parent 5 0 R >> endobj /Resources 354 0 R 8 0 obj /Prev 418 0 R endobj endobj endobj << Evaluation of 14 organic solvents and carriers for screening applications in zebrafish embryos and larvae. >> Whole organisms offer several advantages over cell lines for forward chemical genetic screens, providing information on tissue specificity, toxicity and accounting for bioavailability. >> /D [29 0 R /FitR 52 453 290 453] >> << /Type /Page /D [29 0 R /FitR 52 622 290 622] << /D [28 0 R /FitR 52 572 290 572] /D [27 0 R /FitR 52 219 290 219] /Parent 10 0 R endobj 90 0 obj Univariate analysis was performed by integrating over a wavenumber range corresponding to relevant biomolecules: collagen-rich regions at 918 ± 20 cm, An established model was used in which zebrafish embryos were injected with. /Type /Page 24 0 obj /D [27 0 R /FitR 307 372 545 372] /MediaBox [0 -5.66927 595.276001 785.197021] /CropBox [0 -5.66927 595.276001 785.197021] 128 0 obj 83 0 obj /D [20 0 R /FitR 52 271 290 271] endobj /D [28 0 R /FitR 52 453 290 453] Here, we describe zebrafish-based assays, including both embryo and adult, for evaluation of nanotoxicity caused by metal oxide nanoparticles (NPs), in particular, zinc oxide (ZnO) and titanium oxide (TiO2) nanoparticles. >> >> 89 0 obj endobj /Type /Pages /D [28 0 R /FitR 307 433 545 433] 49 0 obj >> Zebrafish eggs are fertilized and develop outside of the mother's body making visualization with high resolution imaging easier for researchers. << endobj >> >> This review evaluates the potential application and suitability of the zebrafish model in the development of biomaterials and drug delivery systems by investigating the topic of exposure and evaluating test substances on zebrafish embryos and how the FET assay can be applied to evaluate biocompatibility. Brown HK, Schiavone K, Tazzyman S, Heymann D, Chico TJA. With this technique, parental transfer of lipophilic contaminants may be mimicked. >> /Resources 401 0 R << /D [29 0 R /FitR 52 682 290 682] /D [29 0 R /FitR 307 174 545 174] endobj >> Nature. Despite these caveats, several features of zebrafish—including the available genetic resources and transparency of the embryo—have proven useful in a variety of research applications. Phage Therapy Application to Counteract Pseudomonas aeruginosa Infection in Cystic Fibrosis Zebrafish Embryos Authors: Cafora M, Forti F, Briani F, Ghisotti D, Pistocchi A /Count 11 >> induce lesions in zebrafish embryos. The thin endothelial wall of a newly formed vessel is under enormous stress at the onset of blood flow, rapidly acquiring support from mural cells (pericytes and vascular smooth muscle cells; vSMCs) during development. >> endobj endstream Science. For 1 phf embryos, the mortality was 82.3% and this reduced to 34.2% in the 4 hpf group. /Parent 17 0 R (CR80) 109 0 R (CR81) 110 0 R (CR82) 111 0 R (CR83) 112 0 R (CR84) 113 0 R Adult zebrafish (Danio rerio) were maintained according to standard laboratory procedures.19 Embryos and larvae were raised in embryo water (5 mM NaCl, 0.17 mM KCl, 0.33 mM CaCl 2, 0.33 mM MgSO 4) in an incubator at 28.5°C. The results showed disruption in genes associated with muscle development, … 82 0 obj Overall, this study demonstrates the application of confocal Raman spectroscopic imaging for the comparative bimolecular analysis of fully intact and living zebrafish embryos. endobj In the last section we demonstrate … << endobj 4. (CR76) 104 0 R (CR77) 105 0 R (CR78) 106 0 R (CR79) 107 0 R (CR8) 108 0 R endobj /D [27 0 R /FitR 52 102 290 102] 152 0 obj 109 0 obj << << >> endobj << /D [21 0 R /FitR 52 171 290 171] << /ModDate (D:20141113140224+01'00') << /First 170 0 R 105 0 obj /D [28 0 R /FitR 52 632 290 632] Zebrafish are a commonly employed model organism for vertebrate development, 8 disease modeling, 9 and drug discovery. /D [28 0 R /FitR 52 393 290 393] /D [21 0 R /FitR 52 171 290 171] 135 0 obj /MediaBox [0 -5.66927 595.276001 785.197021] << >> 45 0 obj 164 0 obj Fig. We then give an overview of new FCS modalities that are designed to cope with emerging challenges in intracellular applications, e.g. De Luca, E. et al. 58 0 obj 13 0 obj /D [27 0 R /FitR 307 692 545 692] /MediaBox [0 -5.66927 595.276001 785.197021] Epub 2018 Jul 27. 69 0 obj >> -. /Outlines 4 0 R >> /Thumb 282 0 R Position of 10x images relative to 1 dpf zebrafish embryo. 126 0 obj << endobj << << endobj endobj << >> /Next 17 0 R << /Last 171 0 R /ca .2 << (CR59) 85 0 R (CR6) 86 0 R (CR60) 87 0 R (CR61) 88 0 R (CR62) 89 0 R correction of cell membrane movement during measurement and correction of spectral cross-talk of fluorescent proteins. >> >> 101 0 obj Zebrafish-Mycobacterium marinum model for mycobacterial pathogenesis. << /G12 175 0 R >> << endobj /Title /D [28 0 R /FitR 307 712 545 712] Position of 10x images relative to 1 dpf zebrafish embryo. /Contents 404 0 R endobj << /F4 178 0 R endobj (CR9) 119 0 R (CR90) 120 0 R (CR91) 121 0 R (CR92) 122 0 R (CR93) 123 0 R A common approach to unravel the gene networks that drive biology is to examine the consequences of manipulating genes. endobj >> 130 0 obj See this image and copyright information in PMC. 138 0 obj endobj endobj endobj endobj Hence, this review intends to provide a comprehensive account of available scientific evidence of the application of the zebrafish embryos to assess the herbal toxicity. /D [19 0 R /FitR 52 421 290 421] << >> << << 2012;7(10 ):e43850. /Names [(Section16) 158 0 R (Section17) 159 0 R (Section18) 160 0 R (Section19) 161 0 R (Section210) 162 0 R /Contents [410 0 R 411 0 R] Fig. << 60 0 obj /LC 0 << /Producer (Acrobat Distiller 6.0 \(Windows\)) << /D [27 0 R /FitR 307 612 545 612] /TrimBox [0 -5.66927 595.276001 785.197021] 95 0 obj /Thumb 402 0 R >> endobj endobj << /Width 320 << 33 0 obj endobj Simple heart-rate measurement in zebrafish can provide predictive information regarding interactions of chemicals with the components of the cardiac functional regulatory network 15. /Parent 10 0 R /D [20 0 R /FitR 307 246 545 246] >> 37 0 obj << >> The exposure of zebrafish embryos was performed according to the exposure scenario described for the fish embryo acute toxicitytestwiththeexceptionof10insteadof5embryosper glass vial. /D [23 0 R /FitR 0 786 596 0] /Type /Page << 274 0 R 275 0 R 276 0 R 277 0 R 278 0 R 279 0 R] 115 0 obj Zebrafish larvae up to 8 days of post fertilization (dpf) are suitable for high throughput screening because they can grow without nutrient supply. /D [29 0 R /FitR 52 194 290 194] Infection of zebrafish embryos with intracellular bacterial pathogens. 168 0 obj /Rotate 0 By manipulating the local temperature within an embryo, spatial control of transgene expression is also possible. << NLM << 129 0 obj endobj 3. /Annots [182 0 R 183 0 R 184 0 R 185 0 R 186 0 R 187 0 R 188 0 R 189 0 R 190 0 R 191 0 R have been recognized as causative agents of life-threatening systemic infections, primarily in… Read article Nov 25, 2016 Abstract Stereo Microscopy. 44 0 obj /D [29 0 R /FitR 52 323 290 323] /D [29 0 R /FitR 52 523 290 523] Automated Microscopy for Zebrafish And Other Whole Organism Screening Applications. /MediaBox [0 -5.66927 595.276001 785.197021] Zebrafish xenograft models of cancer and metastasis for drug discovery. << /D [22 0 R /FitR 0 786 596 0] >> endobj >> >> Commun.6, 7790 (2015). << 85 0 obj doi: 10.1080/17460441.2017.1297416. /Limits [(Section16) (maintitle)] 88 0 obj /Dest (Section16) /Type /XObject << endobj << endobj /BM /Normal Figure 2. 2008-07-15T06:51:23+08:00 /BitsPerComponent 8 endobj endobj << << %���� /Limits [(CR1) (CR66)] << Expert Opin. /D [20 0 R /FitR 0 786 596 0] /Rotate 0 /Resources 324 0 R /Title () /Resources 391 0 R << /Prev 417 0 R (CR22) 45 0 R (CR23) 46 0 R (CR24) 47 0 R (CR25) 48 0 R (CR26) 49 0 R 43 0 obj /Dest (Section17) << << 154 0 obj << /D [29 0 R /FitR 307 413 545 413] 9 0 obj 35 0 obj Confocal Raman spectroscopic imaging analysis of zebrafish embryo model of tuberculosis. Jahr, W., Schmid, B., Schmied, C., Fahrbach, F. O. /Pages 5 0 R << 123 0 obj /StructParents 0 /D [27 0 R /FitR 307 182 545 182] stream 5. endobj CryoLetters. /D [27 0 R /FitR 52 219 290 219] endobj >> << /Type /Page Mycobacterium marinum infection of adult zebrafish causes caseating granulomatous tuberculosis and is moderated by adaptive immunity. << << /Prev 16 0 R /D [27 0 R /FitR 52 171 290 171] (CR40) 65 0 R (CR41) 66 0 R (CR42) 67 0 R (CR43) 68 0 R (CR44) 69 0 R 64 0 obj >> -. << 336 0 R 337 0 R] >> >> /Count -6 81 0 obj Embryos were embedded and imaged using a M205 FA stereomicro-scope (Leica, Germany). /Dest (Section15) >> << 61 0 obj 293 0 R 294 0 R 295 0 R 296 0 R 297 0 R 298 0 R 299 0 R 300 0 R 301 0 R 302 0 R /Contents 400 0 R 71 0 obj The zebrafish were handled in accordance with Guangdong state regulations on laboratory animal management 2003 Aug 29;225(2):177-82. doi: 10.1016/S0378-1097(03)00446-4. >> << endobj Nano-HA aggregated into the biggest particles around the membrane protein and then caused a little toxicity to development of zebrafish embryos. << /D [19 0 R /FitR 52 495 290 495] endobj 122 0 obj << >> 157 0 obj 159 0 obj uuid:fbae0592-01de-4f93-afdb-94400ed6fd65 171 0 obj << /D [28 0 R /FitR 52 483 290 483] /Count 12 38 0 obj >> >> >> /D [28 0 R /FitR 52 543 290 543] >> /D [29 0 R /FitR 307 134 545 134] /Resources 281 0 R << 121 0 obj endobj endobj << 76 0 obj Time-lapse imaging beyond the diffraction limit. /D [29 0 R /FitR 307 732 545 732] endobj /Last 9 0 R endobj >> >> 104 0 obj 40 0 obj /EmbeddedFiles 8 0 R /D [21 0 R /FitR 307 682 545 682] >> >> >> Would you like email updates of new search results? endobj endobj >> /Parent 10 0 R /F5 179 0 R << /D [27 0 R /FitR 307 712 545 712] >> << Solvent Violet 47 (SV47) and Disperse Blue 14 (DB14) are two anthraquinone dyes that were previously used in different formulations for the production of violet-colored smoke. 17 0 obj >> << >> /CropBox [0 -5.66927 595.276001 785.197021] 2006 Nov;74(11):6108-17. doi: 10.1128/IAI.00887-06. /Font << << << << /Rotate 0 /D [27 0 R /FitR 307 272 545 272] /Dest (Section12) /Title /TrimBox [0 -5.66927 595.276001 785.197021] << 103 0 obj << /D [28 0 R /FitR 52 264 290 264] Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. << Beacon is created at the end of the yolk sac so that the resulting image is set around the trunk of the embryo. endobj The application of a chemical dispersant increased the acute toxicity most likely due to a higher bioavailability of dissolved and … /Contents 375 0 R endobj << /Filter /FlateDecode 113 0 obj (CR36) 60 0 R (CR37) 61 0 R (CR38) 62 0 R (CR39) 63 0 R (CR4) 64 0 R

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